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pmemerald h2b c 10  (Addgene inc)


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    Structured Review

    Addgene inc pmemerald h2b c 10
    Pmemerald H2b C 10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/memerald+paxillin/mEmerald-Paxillin-22+(Plasmid+%2354219)/pmc08684732-197-51-52
    Average 92 stars, based on 8 article reviews
    pmemerald h2b c 10 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Fluctuation-Based Super-Resolution Traction Force Microscopy
    Article Snippet: .. The pmKate2-paxillin vector was purchased from Evrogen (cat.# Page 11 of 33 ACS Paragon Plus Environment Nano Letters 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 12 FP323). mEmerald-Paxillin-22 was a gift from Michael Davidson (Addgene plasmid # 54219) 47. psPAX2 and pMD2.G were gifts from D. Trono (École polytechnique fédérale de Lausanne, Lausanne, Switzerland; Addgene plasmid #12260 and #12259). pCDH-LifeActmRFP was a gift from P. Caswell (University of Manchester, UK). .. AICSDP-1:PXN-EGFP was a gift from The Allen Institute for Cell Science (Addgene plasmid # 87420).

    Article Title: Fluctuation-Based Super-Resolution Traction Force Microscopy
    Article Snippet: .. The pmKate2-paxillin vector was purchased from Evrogen (cat.# FP323). mEmerald-Paxillin-22 was a gift from Michael Davidson (Addgene plasmid # 54219). psPAX2 and pMD2.G were gifts from D. Trono (École polytechnique fédérale de Lausanne, Lausanne, Switzerland; Addgene plasmid #12260 and #12259). pCDH-LifeAct-mRFP was a gift from P. Caswell (University of Manchester, UK). .. AICSDP-1:PXN-EGFP was a gift from The Allen Institute for Cell Science (Addgene plasmid # 87420).

    Article Title: SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras
    Article Snippet: PLA signals were detected with a spinning disc confocal microscope (Marianas spinning disk imaging system, Intelligent Imaging Innovations, Inc.) using 100x objective (NA 1.4 Oil, Plan-Apochromat, M27) and analysed with NIH ImageJ. .. To analyse the dynamics of focal adhesions, cells were transfected with mEmerald-Paxillin (a gift from Michael Davidson, Addgene plasmid # 54219) and plated for at least 6 h on fibronectin-collagen before being imaged live on a TIRF microscope, at 37°C, in presence of 5% CO 2 . ..

    Transfection:

    Article Title: SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras
    Article Snippet: PLA signals were detected with a spinning disc confocal microscope (Marianas spinning disk imaging system, Intelligent Imaging Innovations, Inc.) using 100x objective (NA 1.4 Oil, Plan-Apochromat, M27) and analysed with NIH ImageJ. .. To analyse the dynamics of focal adhesions, cells were transfected with mEmerald-Paxillin (a gift from Michael Davidson, Addgene plasmid # 54219) and plated for at least 6 h on fibronectin-collagen before being imaged live on a TIRF microscope, at 37°C, in presence of 5% CO 2 . ..

    Microscopy:

    Article Title: SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras
    Article Snippet: PLA signals were detected with a spinning disc confocal microscope (Marianas spinning disk imaging system, Intelligent Imaging Innovations, Inc.) using 100x objective (NA 1.4 Oil, Plan-Apochromat, M27) and analysed with NIH ImageJ. .. To analyse the dynamics of focal adhesions, cells were transfected with mEmerald-Paxillin (a gift from Michael Davidson, Addgene plasmid # 54219) and plated for at least 6 h on fibronectin-collagen before being imaged live on a TIRF microscope, at 37°C, in presence of 5% CO 2 . ..



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    Focal adhesion kinetics in mEmerald-paxillin-labelled siControl and siMASTL MDA-MB-231 cells. Time-lapse image sequences taken at 1-min intervals. Video corresponds to <xref ref-type=Fig. 7 D . Frame rate of the video is 7 frames per second. " width="250" height="auto" />
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    Image Search Results


    Focal adhesion kinetics in mEmerald-paxillin-labelled siControl and siMASTL MDA-MB-231 cells. Time-lapse image sequences taken at 1-min intervals. Video corresponds to <xref ref-type=Fig. 7 D . Frame rate of the video is 7 frames per second. " width="100%" height="100%">

    Journal: The Journal of Cell Biology

    Article Title: MASTL promotes cell contractility and motility through kinase-independent signaling

    doi: 10.1083/jcb.201906204

    Figure Lengend Snippet: Focal adhesion kinetics in mEmerald-paxillin-labelled siControl and siMASTL MDA-MB-231 cells. Time-lapse image sequences taken at 1-min intervals. Video corresponds to Fig. 7 D . Frame rate of the video is 7 frames per second.

    Article Snippet: mEmerald-paxillin-22 , A kind gift from Prof. Robert Grosse, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Freiburg and CIBSS – Center for Integrative Biological Signalling Studies, Freiburg, Germany (plasmid 54219; Addgene; http://n2t.net/addgene:54219 ; RRID:Addgene_54219) , ( ) .

    Techniques:

    Antibodies, siRNAs, primers, and DNA constructs used in this study

    Journal: The Journal of Cell Biology

    Article Title: MASTL promotes cell contractility and motility through kinase-independent signaling

    doi: 10.1083/jcb.201906204

    Figure Lengend Snippet: Antibodies, siRNAs, primers, and DNA constructs used in this study

    Article Snippet: mEmerald-paxillin-22 , A kind gift from Prof. Robert Grosse, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Freiburg and CIBSS – Center for Integrative Biological Signalling Studies, Freiburg, Germany (plasmid 54219; Addgene; http://n2t.net/addgene:54219 ; RRID:Addgene_54219) , ( ) .

    Techniques: Construct, Western Blot, Immunofluorescence, Recombinant, Negative Control, Sequencing, Plasmid Preparation